【纳昂达】IGTR Panel v1.0
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DNA 捕获表现
图 1. IGTR Panel v1.0 对不同 gDNA 文库的捕获表现。利用 NadPrep® DNA 通用型文库构建试剂盒 (for Illumina®) 搭配 NadPrep® UDI Adapter (for Illumina®) 构建文库，以 IGTR Panel v1.0 完成杂交捕获。测序模式为 Illumina Novaseq 6000，PE150，利用 BWA 比对到参考基因组 hg38，On-target 按照 reads 数计算。 A. 捕获数据比对率和捕获效率；B. 靶区域覆盖度。
注：样本 gDNA_1-3 分别为： Ramos 细胞系 gDNA 、 Jurkat 细胞系 gDNA 和人类男性基因组 DNA (Promega-male, G1471)。
RNA 捕获表现
图 2. IGTR Panel v1.0 对不同 RNA 文库的捕获表现。利用 NadPrep®Total RNA-To-DNA Module 搭配 NadPrep® DNA 通用型文库构建试剂盒 (for Illumina®>) 建库，以 IGTR Panel v1.0 完成杂交捕获。测序模式为 Illumina Novaseq 6000，PE150，salmon 进行分析。
注：样本 RNA_1-3 分别为： Ramos 细胞系总 RNA、 Jurkat 细胞系总 RNA 和外周血白细胞总 RNA (Takara, 636592)。
RNA 捕获转录本覆盖示例
Ramos 细胞
Jurkat 细胞
图 3. IGTR Panel v1.0 对不同细胞 RNA 捕获转录本的覆盖示例。
捕获灵敏度表现
图 4. IGTR Panel v1.0 对 DNA 和 RNA 文库捕获的灵敏度表现。利用 NadPrep® Total RNA-To-DNA Module 搭配 NadPrep® DNA 通用型文库构建试剂盒 (forIllumina®) 建库，以 IGTR Panel v1.0 完成杂交捕获。测序模式为 Illumina Novaseq 6000，PE150。
注： Ramos+Jurkat DNA 以人类男性基因组 DNA (Promega-male, G1471) 梯度稀释；Ramos+Jurkat RNA 以外周血白细胞总 RNA (TAKARA, 636592) 梯度稀释。
基因列表
IG 编码区覆盖
|
IGHV1-2 |
IGHV1-3 |
IGHV1-18 |
IGHV1-24 |
IGHV1-45 |
IGHV1-46 |
IGHV1-58 |
IGHV1-69 |
IGHV1-69-2 |
IGHV1-69D |
|
IGHV1OR15-1 |
IGHV1OR21-1 |
IGHV1OR16-2 |
IGHV1OR15-9 |
IGHV2-5 |
IGHV2-26 |
IGHV2-70 |
IGHV2-70D |
IGHV2OR16-5 |
IGHV3-7 |
|
IGHV3-11 |
IGHV3-13 |
IGHV3-15 |
IGHV3-16 |
IGHV3-20 |
IGHV3-21 |
IGHV3-23 |
IGHV3-25 |
IGHV3-30 |
IGHV3-33 |
|
IGHV3-35 |
IGHV3-38 |
IGHV3-43 |
IGHV3-48 |
IGHV3-49 |
IGHV3-53 |
IGHV3-62 |
IGHV3-64 |
IGHV3-66 |
IGHV3-72 |
|
IGHV3-73 |
IGHV3-74 |
IGHV3-64D |
IGHV3OR16-6 |
IGHV3OR15-7 |
IGHV3OR16-8 |
IGHV3OR16-9 |
IGHV3OR16-10 |
IGHV3OR16-12 |
IGHV3OR16-13 |
|
IGHV3OR16-17 |
IGHV4-4 |
IGHV4-28 |
IGHV4-31 |
IGHV4-34 |
IGHV4-39 |
IGHV4-59 |
IGHV4-61 |
IGHV4OR15-8 |
IGHV5-51 |
|
IGHV5-10-1 |
IGHV6-1 |
IGHV7-81 |
IGHV7-4-1 |
IGHV8-51-1 |
IGHD1-1 |
IGHD1-7 |
IGHD1-14 |
IGHD1-20 |
IGHD1-26 |
|
IGHD1OR15-1A |
IGHD1OR15-1B |
IGHD2-2 |
IGHD2-8 |
IGHD2-15 |
IGHD2-21 |
IGHD2OR15-2A |
IGHD2OR15-2B |
IGHD3-3 |
IGHD3-9 |
|
IGHD3-10 |
IGHD3-16 |
IGHD3-22 |
IGHD3OR15-3A |
IGHD3OR15-3B |
IGHD4-4 |
IGHD4-11 |
IGHD4-17 |
IGHD4-23 |
IGHD4OR15-4A |
|
IGHD4OR15-4B |
IGHD5-5 |
IGHD5-12 |
IGHD5-18 |
IGHD5-24 |
IGHD5OR15-5A |
IGHD5OR15-5B |
IGHD6-6 |
IGHD6-13 |
IGHD6-19 |
|
IGHD6-25 |
IGHD7-27 |
IGHJ1 |
IGHJ2 |
IGHJ3 |
IGHJ4 |
IGHJ5 |
IGHJ6 |
IGHA1 |
IGHA2 |
|
IGHD |
IGHE |
IGHG1 |
IGHG2 |
IGHG3 |
IGHG4 |
IGHGP |
IGHM |
IGKV1-5 |
IGKV1-6 |
|
IGKV1-8 |
IGKV1D-8 |
IGKV1-9 |
IGKV1-12 |
IGKV1D-12 |
IGKV1-13 |
IGKV1D-13 |
IGKV1-16 |
IGKV1D-16 |
IGKV1-17 |
|
IGKV1D-17 |
IGKV1-27 |
IGKV1-33 |
IGKV1D-33 |
IGKV1-37 |
IGKV1D-37 |
IGKV1-39 |
IGKV1D-39 |
IGKV1D-42 |
IGKV1D-43 |
|
IGKV1OR2-108 |
IGKV2-24 |
IGKV2D-24 |
IGKV2D-26 |
IGKV2-28 |
IGKV2D-28 |
IGKV2-29 |
IGKV2D-29 |
IGKV2-30 |
IGKV2D-30 |
|
IGKV2-40 |
IGKV2D-40 |
IGKV3-7 |
IGKV3D-7 |
IGKV3-11 |
IGKV3D-11 |
IGKV3-15 |
IGKV3D-15 |
IGKV3-20 |
IGKV3D-20 |
|
IGKV3OR2-268 |
IGKV4-1 |
IGKV5-2 |
IGKV6-21 |
IGKV6D-21 |
IGKV6D-41 |
IGKJ1 |
IGKJ2 |
IGKJ3 |
IGKJ4 |
|
IGKJ5 |
IGKC |
IGLV1-36 |
IGLV1-40 |
IGLV1-41 |
IGLV1-44 |
IGLV1-47 |
IGLV1-50 |
IGLV1-51 |
IGLV2-8 |
|
IGLV2-11 |
IGLV2-14 |
IGLV2-18 |
IGLV2-23 |
IGLV2-33 |
IGLV3-1 |
IGLV3-9 |
IGLV3-10 |
IGLV3-12 |
IGLV3-16 |
|
IGLV3-19 |
IGLV3-21 |
IGLV3-22 |
IGLV3-25 |
IGLV3-27 |
IGLV3-32 |
IGLV4-3 |
IGLV4-60 |
IGLV4-69 |
IGLV5-37 |
|
IGLV5-45 |
IGLV5-48 |
IGLV5-52 |
IGLV6-57 |
IGLV7-43 |
IGLV7-46 |
IGLV8-61 |
IGLV8OR8-1 |
IGLV9-49 |
IGLV10-54 |
|
IGLV11-55 |
IGLJ1 |
IGLJ2 |
IGLJ3 |
IGLJ4 |
IGLJ5 |
IGLJ6 |
IGLJ7 |
IGLC1 |
IGLC2 |
|
IGLC3 |
IGLC6 |
IGLC7 |
IGLL1 |
IGLL5 |
TR 编码区覆盖
|
TRAV1-1 |
TRAV1-2 |
TRAV2 |
TRAV3 |
TRAV4 |
TRAV5 |
TRAV6 |
TRAV7 |
TRAV8-1 |
TRAV8-2 |
|
TRAV8-3 |
TRAV8-4 |
TRAV8-6 |
TRAV8-7 |
TRAV9-1 |
TRAV9-2 |
TRAV10 |
TRAV12-1 |
TRAV12-2 |
TRAV12-3 |
|
TRAV13-1 |
TRAV13-2 |
TRAV14DV4 |
TRAV16 |
TRAV17 |
TRAV18 |
TRAV19 |
TRAV20 |
TRAV21 |
TRAV22 |
|
TRAV23DV6 |
TRAV24 |
TRAV25 |
TRAV26-1 |
TRAV26-2 |
TRAV27 |
TRAV29DV5 |
TRAV30 |
TRAV34 |
TRAV35 |
|
TRAV36DV7 |
TRAV38-1 |
TRAV38-2DV8 |
TRAV39 |
TRAV40 |
TRAV41 |
TRAJ1 |
TRAJ2 |
TRAJ3 |
TRAJ4 |
|
TRAJ5 |
TRAJ6 |
TRAJ7 |
TRAJ8 |
TRAJ9 |
TRAJ10 |
TRAJ11 |
TRAJ12 |
TRAJ13 |
TRAJ14 |
|
TRAJ15 |
TRAJ16 |
TRAJ17 |
TRAJ18 |
TRAJ19 |
TRAJ20 |
TRAJ21 |
TRAJ22 |
TRAJ23 |
TRAJ24 |
|
TRAJ25 |
TRAJ26 |
TRAJ27 |
TRAJ28 |
TRAJ29 |
TRAJ30 |
TRAJ31 |
TRAJ32 |
TRAJ33 |
TRAJ34 |
|
TRAJ35 |
TRAJ36 |
TRAJ37 |
TRAJ38 |
TRAJ39 |
TRAJ40 |
TRAJ41 |
TRAJ42 |
TRAJ43 |
TRAJ44 |
|
TRAJ45 |
TRAJ46 |
TRAJ47 |
TRAJ48 |
TRAJ49 |
TRAJ50 |
TRAJ52 |
TRAJ53 |
TRAJ54 |
TRAJ56 |
|
TRAJ57 |
TRAJ58 |
TRAJ59 |
TRAJ61 |
TRAC |
TRBV2 |
TRBV3-1 |
TRBV4-1 |
TRBV4-2 |
TRBV5-1 |
|
TRBV5-3 |
TRBV5-4 |
TRBV5-5 |
TRBV5-6 |
TRBV5-7 |
TRBV6-1 |
TRBV6-2 |
TRBV6-4 |
TRBV6-5 |
TRBV6-6 |
|
TRBV6-7 |
TRBV6-8 |
TRBV7-1 |
TRBV7-2 |
TRBV7-3 |
TRBV7-4 |
TRBV7-6 |
TRBV7-7 |
TRBV7-9 |
TRBV9 |
|
TRBV10-1 |
TRBV10-2 |
TRBV10-3 |
TRBV11-1 |
TRBV11-2 |
TRBV11-3 |
TRBV12-3 |
TRBV12-4 |
TRBV12-5 |
TRBV13 |
|
TRBV14 |
TRBV15 |
TRBV16 |
TRBV17 |
TRBV18 |
TRBV19 |
TRBV20-1 |
TRBV20OR9-2 |
TRBV23-1 |
TRBV23OR9-2 |
|
TRBV24-1 |
TRBV24OR9-2 |
TRBV25-1 |
TRBV27 |
TRBV28 |
TRBV29-1 |
TRBV29OR9-2 |
TRBV30 |
TRBD1 |
TRBD2 |
|
TRBJ1-1 |
TRBJ1-2 |
TRBJ1-3 |
TRBJ1-4 |
TRBJ1-5 |
TRBJ1-6 |
TRBJ2-1 |
TRBJ2-2 |
TRBJ2-3 |
TRBJ2-4 |
|
TRBJ2-5 |
TRBJ2-6 |
TRBJ2-7 |
TRBJ2-2P |
TRBC1 |
TRBC2 |
TRGV1 |
TRGV2 |
TRGV3 |
TRGV4 |
|
TRGV5 |
TRGV8 |
TRGV9 |
TRGV10 |
TRGV11 |
TRGJ1 |
TRGJ2 |
TRGJP1 |
TRGJP2 |
TRGJP |
|
TRGC1 |
TRGC2 |
TRDV1 |
TRDV2 |
TRDV3 |
TRDD1 |
TRDD2 |
TRDD3 |
TRDJ1 |
TRDJ2 |
|
TRDJ3 |
TRDJ4 |
TRDC |
仅限研究使用，不用于临床诊断。
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